One group of sheep remains undrenched throughout the trial, and is known at the control group. Trial sheep should be run on the same paddock from which they came for the whole trial (10-14 days). They can run with the main mob, even if these have been treated.
Step 1: Draft off a sufficient number of even-sized weaners from the mob.
Step 2: To allocate sheep to groups, run them into a race and sequentially mark heads with appropriate colour(s) on the forehead. Proceed down the race until all sheep have been marked.
Step 3: Run sheep through the race and draft into groups. Return the control group to main mob.
Step 4: Using scales weigh a few of the largest sheep. Calculate the dose required for each drench based on the heaviest weaner in the mob.
Step 5: Prepare drenches and check gun delivery. Set gun to deliver correct dose. Squirt 10 doses of the first drench to be administered into measuring cylinder, divide total volume by 10 to give output of gun. Adjust gun and repeat procedure with second drench gun to ensure both deliver correct dose. Preferably use two guns and backpacks. To minimise contamination wash gun and pack and check dose and gun delivery when changing drenches.
Step 6: Return each group of 15 sheep to the race and administer the drench(es) for that group. Take care that each sheep in the group is treated.
Step 7: Repeat until all groups of sheep have been treated.
Step 8: Return all weaners to the main mob.
Step 9: 10-14 days later collect faecal samples. Muster mob early in the morning. Draft marked lambs into colour groups. Minimise stress, it is difficult to collect adequate samples from stressed lambs.
Step 10. Collect individual dung samples from 10 of the lambs in each group, a minimum of 10-15 pellets per sample. Samples are collected from the rectum of each sheep using the rubber glove provided with the kit, and put into the appropriate containers. Keep samples cool and ensure delivery to the testing laboratory in a timely manner.